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ERMN Polyclonal antibody

ERMN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31432-1-AP

Synonyms

ermin, ermin, ERM like protein, ERMN, JN, Juxtanodin, KIAA1189

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERMN Polyclonal antibody (31432-1-AP)

    WB analysis using 31432-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31432-1-AP (ERMN antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inrat brain tissue, rat cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31432-1-AP targets ERMN in WB, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERMN fusion protein Ag35648
Full Name ermin, ERM-like protein
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC026345
Gene Symbol ERMN
Gene ID (NCBI) 57471
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERMN is associated with cytoskeletal rearrangements and the stability of myelin sheath. A study has shown that ERMN is expressed specifically in oligodendrocytes in the central nervous system (CNS), and is involved in the formation of myelin (PMID: 20934411). The predicted MW of ERMN is 33 KDa, while western blot analyses detected a 45 kDa single band protein as a result of phosphorylation modification.

Protocols

Product Specific Protocols
WB protocol for ERMN antibody 31432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols