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KIAA1715 Polyclonal antibody

KIAA1715 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31308-1-AP

Synonyms

LNP, ER junction formation factor lunapark, ulnaless, Ul, Protein lunapark

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA1715 Polyclonal antibody (31308-1-AP)

    WB analysis using 31308-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 31308-1-AP (KIAA1715 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KIAA1715 Polyclonal antibody (31308-1-AP)

    IHC staining of mouse cerebellum using 31308-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31308-1-AP (KIAA1715 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using KIAA1715 Polyclonal antibody (31308-1-AP)

    IF Staining of U2OS using 31308-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KIAA1715 antibody (31308-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Tested Applications

Positive WB detected inU2OS cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31308-1-AP targets KIAA1715 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA1715 fusion protein Ag34669
Full Name KIAA1715
Observed Molecular Weight51 kDa
GenBank Accession NumberBC105132
Gene Symbol KIAA1715
Gene ID (NCBI) 80856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA1715, also known as LNPK, is a member of the conserved Lunapark protein family. KIAA1715 is an endoplasmic reticulum (ER) membrane protein consisting of an N-terminal domain important for N-myristoylation and ER targeting, two transmembrane domains and a unique zinc finger domain. It has 4 isoforms produced by alternative splicing and acts mainly as a stabilizer of negative membrane curvature (PMID: 27619977).

Protocols

Product Specific Protocols
WB protocol for KIAA1715 antibody 31308-1-APDownload protocol
IHC protocol for KIAA1715 antibody 31308-1-APDownload protocol
IF protocol for KIAA1715 antibody 31308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols