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  • Western Blot (WB) analysis of various lysates using CD3EAP Polyclonal antibody (31014-1-AP)

    WB analysis using 31014-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31014-1-AP (CD3EAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CD3EAP Polyclonal antibody (31014-1-AP)

    IF Staining of U2OS using 31014-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CD3EAP antibody (31014-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD3EAP Polyclonal antibody (31014-1-AP)

    IF Staining of HeLa using 31014-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD3EAP antibody (31014-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

CD3EAP Polyclonal antibody

CD3EAP Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31014-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CD3E-associated protein, CAST, ASE-1, ASE1, Antisense to ERCC-1 protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA431 cells, A549 cells, HeLa cells, NCI-H1299 cells
Positive IF/ICC detected inHeLa cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31014-1-AP targets CD3EAP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD3EAP fusion protein Ag34108 Predict reactive species
Full Name CD3e molecule, epsilon associated protein
Calculated Molecular Weight55 kDa
Observed Molecular Weight80-90 kDa
GenBank Accession NumberNM_012099.1
Gene Symbol CD3EAP
Gene ID (NCBI) 10849
RRIDAB_3669815
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO15446
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 and as CD3ɛ-associated signal transducer (CAST), CD3EAP is a 55 kDa nucleolar autoantigen that has an apparent molecular mass of 90 kDa (PMID: 11199923). CD3EAP/hPAF49 contains a single tyrosine residue at position 82 (Tyr82), which is phosphorylated upon stimulation of the T-cell receptor. Western blot analysis detected CD3EAP at an apparent molecular mass of ~80-90 kDa.

Protocols

Product Specific Protocols
WB protocol for CD3EAP antibody 31014-1-APDownload protocol
IF protocol for CD3EAP antibody 31014-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols