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  • Western Blot (WB) analysis of various lysates using COL5A3 Polyclonal antibody (30204-1-AP)

    WB analysis using 30204-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30204-1-AP (COL5A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using COL5A3 Polyclonal antibody (30204-1-AP)

    IF Staining of HUVEC using 30204-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using COL5A3 antibody (30204-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

COL5A3 Polyclonal antibody

COL5A3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30204-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

COL5, Collagen Type V, Collagen alpha-3(V) chain, Collagen alpha 3(V) chain

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, HEK-293T cells
Positive IF/ICC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30204-1-AP targets COL5A3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COL5A3 fusion protein Ag30705 Predict reactive species
Full Name collagen, type V, alpha 3
Calculated Molecular Weight 172 kDa
Observed Molecular Weight145 kDa
GenBank Accession NumberNM_015719
Gene Symbol COL5A3
Gene ID (NCBI) 50509
RRIDAB_3669706
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25940
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COL5A3 (Collagen type V alpha 3) belongs to the fibrillar collagen family. Type V collagen (COLV) is a member of a group I collagen (fibrillar forming collagen) and has three varieties of α chains: α1 (COL5A1), α2 (COL5A2), and α3 (COL5A3). COLV is a minor connective tissue component of nearly ubiquitous distribution. It binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. This antibody is specific to COL5A3.

Protocols

Product Specific Protocols
WB protocol for COL5A3 antibody 30204-1-APDownload protocol
IF protocol for COL5A3 antibody 30204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols