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NUP85 Polyclonal antibody

NUP85 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30158-1-AP

Synonyms

85 kDa nucleoporin, FLJ12549, FROUNT, nucleoporin 85kDa, Nucleoporin Nup75, Nucleoporin Nup85, Nup75, NUP85

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUP85 Polyclonal antibody (30158-1-AP)

    WB analysis using 30158-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30158-1-AP (Pericentrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NUP85 Polyclonal antibody (30158-1-AP)

    WB analysis using 30158-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30158-1-AP (NUP85 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue, mouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30158-1-AP targets NUP85 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUP85 fusion protein Ag33004
Full Name nucleoporin 85kDa
Calculated Molecular Weight 75 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC000697
Gene Symbol NUP85
Gene ID (NCBI) 79902
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nucleoporin 85 (Nup85) (also known as FROUNT) is a protein that in humans is encoded by the NUP85 gene. This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response.

Protocols

Product Specific Protocols
WB protocol for NUP85 antibody 30158-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols