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  • Featured Product
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PLCD4 Polyclonal antibody

PLCD4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30133-1-AP

Synonyms

hPLCD4, Phospholipase C delta 4, phospholipase C, delta 4, PLC delta 4, PLCD4

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PLCD4 Polyclonal antibody (30133-1-AP)

    WB analysis of HeLa using 30133-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 30133-1-AP (PLCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PLCD4 Polyclonal antibody (30133-1-AP)

    WB analysis of HeLa using 30133-1-AP

    WB result of PLCD4 antibody (30133-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLCD4 transfected HeLa cells.

  • Western Blot (WB) analysis of rat testis tissue using PLCD4 Polyclonal antibody (30133-1-AP)

    WB analysis of rat testis using 30133-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 30133-1-AP (PLCD4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PLCD4 Polyclonal antibody (30133-1-AP)

    WB analysis using 30133-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 30133-1-AP (PLCD4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PLCD4 Polyclonal antibody (30133-1-AP)

    IHC staining of mouse testis using 30133-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30133-1-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PLCD4 Polyclonal antibody (30133-1-AP)

    IHC staining of human pancreas cancer using 30133-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30133-1-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, rat testis tissue, human testis tissue
Positive IHC detected inmouse testis tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30133-1-AP targets PLCD4 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLCD4 fusion protein Ag32651
Full Name phospholipase C, delta 4
Calculated Molecular Weight 85-90 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC006355
Gene Symbol PLCD4
Gene ID (NCBI) 84812
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLCD4(1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4), also known as hPLCD4. It is highly expressed in skeletal muscle and kidney tissues. It may be an important enzyme for intracellular Ca²⁺ mobilization in the zona pellucida-induced acrosome reaction. PLCD4 cooperates with nuclear PKC to regulate liver regeneration. Overexpression of PLCD4 upregulates Erk signaling pathway and proliferation (PMID: 15140260).

Protocols

Product Specific Protocols
WB protocol for PLCD4 antibody 30133-1-APDownload protocol
IHC protocol for PLCD4 antibody 30133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols