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CNDP2 Polyclonal antibody

CNDP2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30025-1-AP

Synonyms

CN2, CNDP dipeptidase 2, CNDP2, CPGL, FLJ10830, HsT2298, PEPA, Peptidase A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNDP2 Polyclonal antibody (30025-1-AP)

    WB analysis using 30025-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30025-1-AP (CNDP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293T cells, HL-60 cells, Jurkat cells, PC-3 cells, U-251 cells, U-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30025-1-AP targets CNDP2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNDP2 fusion protein Ag32312
Full Name CNDP dipeptidase 2 (metallopeptidase M20 family)
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 44-53 kDa
GenBank Accession NumberBC001375
Gene Symbol CNDP2
Gene ID (NCBI) 55748
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carnosine dipeptidase II (CNDP2) catalyzes the peptide bond hydrolysis in dipeptides, displaying a non-redundant activity toward threonyl dipeptides. It has high dipeptidase activity toward cysteinyl glycine, an intermediate metabolite in glutathione metabolism (PubMed:19346245, PubMed:12473676). Metabolizes N-lactoyl-amino acids, both through hydrolysis to form lactic acid and amino acids, as well as through their formation by reverse proteolysis (PubMed:25964343). CNDP2 has 2 isoforms with a molecular weight of 53 and 44 kDa. The isoform 1 is ubiquitously expressed with higher levels in the kidney and liver (at protein level) and expressed in peripheral blood leukocytes (PubMed:12473676). It is also expressed in gastric mucosa and down-regulated in gastric cancer mucosal tissues (at the protein level) (PubMed:24395568). Isoform 2 is broadly expressed in fetal tissues and expressed in the adult liver and placenta. (PMID: 17121880).

Protocols

Product Specific Protocols
WB protocol for CNDP2 antibody 30025-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols