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  • Western Blot (WB) analysis of various lysates using M-cadherin Polyclonal antibody (29928-1-AP)

    WB analysis using 29928-1-AP

    C2C12 cells were subjected to SDS PAGE followed by western blot with 29928-1-AP (M-cadherin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using M-cadherin Polyclonal antibody (29928-1-AP)

    IF Staining of mouse skeletal muscle using 29928-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using M-cadherin antibody (29928-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

M-cadherin Polyclonal antibody

M-cadherin Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 29928-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

CDH15, Cadherin 14, Cadherin 15, Cadherin-14, Cadherin-15

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inC2C12 cells
Positive IF-P detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29928-1-AP targets M-cadherin in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen M-cadherin fusion protein Ag25763 Predict reactive species
Full Name cadherin 15, type 1, M-cadherin (myotubule)
Calculated Molecular Weight 89 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC008951
Gene Symbol M-cadherin
Gene ID (NCBI) 1013
RRIDAB_3086192
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55291
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. M-cadherin (muscle cadherin), also known as CDH15 (cadherin-15), is a 124-kDa type I transmembrane glycoprotein that is highly expressed in developing skeletal muscle, satellite cells, and cerebellum (PMID: 9545347; 12052883). It associates with β-catenin and functions as a cell-cell adhesion molecule in a homophilic and specific manner (PMID: 9545347). M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation (PMID: 1840697).

Protocols

Product Specific Protocols
WB protocol for M-cadherin antibody 29928-1-APDownload protocol
IF protocol for M-cadherin antibody 29928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols