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EIF4E Polyclonal antibody

EIF4E Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29712-1-AP

Synonyms

CBP, eIF 4E, eIF 4F 25 kDa subunit, EIF4E, EIF4E1, EIF4EL1, EIF4F, mRNA cap binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF4E Polyclonal antibody (29712-1-AP)

    WB analysis using 29712-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29712-1-AP (EIF4E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EIF4E Polyclonal antibody (29712-1-AP)

    IHC staining of human colon cancer using 29712-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29712-1-AP (EIF4E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EIF4E Polyclonal antibody (29712-1-AP)

    IHC staining of human colon cancer using 29712-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29712-1-AP (EIF4E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF4E Polyclonal antibody (29712-1-AP)

    IF Staining of HepG2 using 29712-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF4E antibody (29712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, HSC-T6 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29712-1-AP targets EIF4E in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name eukaryotic translation initiation factor 4E
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 26-29 kDa
GenBank Accession NumberBC012611
Gene Symbol EIF4E
Gene ID (NCBI) 1977
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic translation initiation factor 4E, also known as eIF4E, is a protein that in humans is encoded by the EIF4E gene. eIF4E is the mRNA cap-binding protein, known as a general initiation factor allowing for mRNA-ribosome interaction and cap-dependent translation in eukaryotic cells. eIF4E is a polypeptide that exists as both a free form and as part of the eIF4F pre-initiation complex. Regulation of eIF4E may be achieved via three distinct mechanisms: transcription, phosphorylation, and inhibitory proteins.

Protocols

Product Specific Protocols
WB protocol for EIF4E antibody 29712-1-APDownload protocol
IHC protocol for EIF4E antibody 29712-1-APDownload protocol
IF protocol for EIF4E antibody 29712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols