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Phospho-PDH E1 Alpha (Ser300) Polyclonal antibody

Phospho-PDH E1 Alpha (Ser300) Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29583-1-AP

Synonyms

PDHA, PDHA1, PDHCE1A, PDHE1 A type I, PHE1A, Phospho-PDH E1 Alpha (Ser300)

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-PDH E1 Alpha (Ser300) Polyclonal antibody (29583-1-AP)

    WB analysis using 29583-1-AP

    10 % fetal bovine serum treated HeLa cells and Serum-free treated HeLa cells were subjected to SDS PAGE followed by western blot with 29583-1-AP (Phospho-PDH E1 Alpha (Ser300) antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Phospho-PDH E1 Alpha (Ser300) Polyclonal antibody (29583-1-AP)

    IF Staining of HepG2 using 29583-1-AP

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HepG2 cells using Phospho-PDH E1 Alpha (Ser300) antibody (29583-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inSerum-free treated HeLa cells, 10 % fetal bovine serum treated HeLa cells
Positive IF/ICC detected inλ phosphatase treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29583-1-AP targets Phospho-PDH E1 Alpha (Ser300) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name pyruvate dehydrogenase (lipoamide) alpha 1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC002406
Gene Symbol PDHA1
Gene ID (NCBI) 5160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)

Protocols

Product Specific Protocols
WB protocol for Phospho-PDH E1 Alpha (Ser300) antibody 29583-1-APDownload protocol
IF protocol for Phospho-PDH E1 Alpha (Ser300) antibody 29583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Dis

Pyruvate dehydrogenase kinase 1 protects against neuronal injury and memory loss in mouse models of diabetes

Authors - Yuan Yao
View Article