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MCP-1/CCL2 Polyclonal antibody

MCP-1/CCL2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29547-1-AP

Synonyms

CCL2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of THP-1 cells using MCP1 Polyclonal antibody (29547-1-AP)

    WB analysis of THP-1 using 29547-1-AP

    Non-treated, and PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29547-1-AP (MCP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inPMA, LPS and Brefeldin A treated THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29547-1-AP targets MCP-1/CCL2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein
Full Name chemokine (C-C motif) ligand 2
Calculated Molecular Weight 99 aa, 11 kDa
Observed Molecular Weight11 kDa
GenBank Accession NumberBC009716
Gene Symbol MCP-1/CCL2
Gene ID (NCBI) 6347
RRIDAB_2918323
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCP-1, ( also named as CCL2) is a member of the chemokine family. MCP-1 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. MCP-1 has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. Elevated expression of MCP-1 is associated with severe acute respiratory syndrome coronavirus 2 infection.

Protocols

Product Specific Protocols
WB protocol for MCP-1/CCL2 antibody 29547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Ethnopharmacol

Transcriptomic analysis reveals the critical role of Chemokine signaling in the anti-atherosclerosis effect of Xuefu Zhuyu Decoction

Authors - Dongdong Jia
View Article