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CRMP1 Polyclonal antibody

CRMP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29497-1-AP

Synonyms

CRMP 1, CRMP1, DPYSL1, DRP 1, DRP1, ULIP 3, ULIP3, Unc 33 like phosphoprotein 3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CRMP1 Polyclonal antibody (29497-1-AP)

    WB analysis using 29497-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 29497-1-AP (CRMP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRMP1 Polyclonal antibody (29497-1-AP)

    IHC staining of mouse brain using 29497-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29497-1-AP (CRMP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRMP1 Polyclonal antibody (29497-1-AP)

    IHC staining of mouse brain using 29497-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29497-1-AP (CRMP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse lung tissue, rat lung tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29497-1-AP targets CRMP1 in WB, IHC, ELISA applications and shows reactivity with Mouse, Rat samples.

Tested Reactivity Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRMP1 fusion protein Ag31559
Full Name collapsin response mediator protein 1
Calculated Molecular Weight62KD
Observed Molecular Weight62 kDa
GenBank Accession NumberNM_001014809
Gene Symbol CRMP1
Gene ID (NCBI) 1400
RRIDAB_2918317
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRMP1 (Collapsin response mediator protein 1) is also named as DPYSL1, ULIP3, DRP-1 and belongs to the collapsin response mediator protein family, which are a family of five cytoplasmic proteins predominantly expressed in the developing nervous system. CRMP1, the predicted 62-kDa protein and its 75-kDa isoform corresponding to its N-terminal splice variant already described for embryonic, neonatal, and adult brain of the rat are evidenced in the cortex of adult mouse (PMID:15834957). This protein is involved in invasion, and aggressiveness of prolactin pituitary tumors.

Protocols

Product Specific Protocols
WB protocol for CRMP1 antibody 29497-1-APDownload protocol
IHC protocol for CRMP1 antibody 29497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols