• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Phospho-GRB10 (Ser476) Polyclonal antibody

Phospho-GRB10 (Ser476) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28873-1-AP

Synonyms

Grb 10, GRB IR, GRB10, GRB10 adapter protein, GRBIR, IRBP, KIAA0207, MEG1, Phospho-GRB10 (Ser476), RSS

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-GRB10 (Ser476) Polyclonal antibody (28873-1-AP)

    WB analysis using 28873-1-AP

    Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28873-1-AP (Phospho-GRB10 (Ser476) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

Tested Applications

Positive WB detected inλ phosphatase treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28873-1-AP targets Phospho-GRB10 (Ser476) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name growth factor receptor-bound protein 10
Calculated Molecular Weight536aa,61 kDa; 594aa,67 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC024285
Gene Symbol GRB10
Gene ID (NCBI) 2887
RRIDAB_2918210
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Growth factor receptor-bound protein 10 (GRB10) is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including the INSR and INS-like growth factor (IGF1R) receptors. It may play a role in mediating INS-stimulated ubiquitination of INSR, leading to proteasomal degradation. It has been reported that GRB10 is a direct substrate of mechanistic/mammalian target of mTOR and can be phosphorylated at Ser150, Ser428 and Ser476 identified as MAPK-mediated phosphorylation sites. (PMID: 15952796, 24746805, 21659605)

Protocols

Product Specific Protocols
WB protocol for Phospho-GRB10 (Ser476) antibody 28873-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols