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SARS-CoV-2 Nucleocapsid Phosphoprotein Polyclonal antibody

SARS-CoV-2 Nucleocapsid Phosphoprotein Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Virus and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28769-1-AP

Synonyms

N protein, nucleocapsid phosphoprotein

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SARS-CoV-2 Nucleocapsid Phosphoprotein Polyclonal (28769-1-AP)

    WB analysis using 28769-1-AP

    SARS-CoV-2 Nucleocapsid Phosphoprotein with the domain 1-419aa expressed in HEK-293 cells were subjected to SDS PAGE followed by western blot with 28769-1-AP (2019-nCOV nucleocapsid phosphoprotein antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • ELISA experiment of Recombinant protein using SARS-CoV-2 Nucleocapsid Phosphoprotein Polyclonal (28769-1-AP)

    ELISA experiment of Recombinant protein using 28769-1-AP

    SARS-CoV-2 Nucleocapsid Phosphoprotein Antibody (28769-1-AP) tested by ELISA.SARS-CoV-2 Nucleocapsid Phosphoprotein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Nucleocapsid Phosphoprotein Antibody (28769-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

Tested Applications

Positive WB detected inRecombinant protein
Positive ELISA detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

28769-1-AP targets SARS-CoV-2 Nucleocapsid Phosphoprotein in WB, ELISA applications and shows reactivity with Virus samples.

Tested Reactivity Virus
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676
Full Name COVID-19 N Protein
GenBank Accession NumberNC_045512
Gene Symbol COVID-19 N Protein
Gene ID (NCBI) 43740575
RRIDAB_2881212
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity.(PMID: 32416961, PMID: 32235387)

Protocols

Product Specific Protocols
WB protocol for SARS-CoV-2 Nucleocapsid Phosphoprotein antibody 28769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

SARS-CoV-2 NSP5 and N protein counteract the RIG-I signaling pathway by suppressing the formation of stress granules.

Authors - Yi Zheng
View Article
humanWB

Antiviral Res

RBM24 inhibits the translation of SARS-CoV-2 polyproteins by targeting the 5ʹ-untranslated region

Authors - Yongxuan Yao
View Article
humanWB

Virol J

LINC01002 functions as a ceRNA to regulate FRMD8 by sponging miR-4324 for the development of COVID-19

Authors - Xinyi Kong
View Article