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VPS33B Polyclonal antibody

VPS33B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28653-1-AP

Synonyms

FLJ14848, hVPS33B, VPS33B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS33B Polyclonal antibody (28653-1-AP)

    WB analysis using 28653-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28653-1-AP (VPS33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using VPS33B Polyclonal antibody (28653-1-AP)

    IHC staining of mouse kidney using 28653-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28653-1-AP (VPS33B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using VPS33B Polyclonal antibody (28653-1-AP)

    IHC staining of mouse kidney using 28653-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28653-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VPS33B Polyclonal antibody (28653-1-AP)

    IHC staining of mouse brain using 28653-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28653-1-AP (VPS33B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VPS33B Polyclonal antibody (28653-1-AP)

    IHC staining of mouse brain using 28653-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28653-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28653-1-AP targets VPS33B in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS33B fusion protein Ag29886
Full Name vacuolar protein sorting 33 homolog B (yeast)
Calculated Molecular Weight 617 aa, 71 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC016445
Gene Symbol VPS33B
Gene ID (NCBI) 26276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS33B, a homolog of yeast class C vacuolar protein sorting (vps) protein Vps33p, belongs to the STXBP/unc-18/SEC1 family. It may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. VPS33B mediates phagolysosomal fusion in macrophages (PMID: 18474358). Defects in VPS33B account for most cases of arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).

Protocols

Product Specific Protocols
WB protocol for VPS33B antibody 28653-1-APDownload protocol
IHC protocol for VPS33B antibody 28653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols