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BCAT1 Polyclonal antibody

BCAT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28622-1-AP

Synonyms

BCAT1, DKFZp686E12175, ECA39, MECA39, PNAS 121, PP18, Protein ECA39

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCAT1 Polyclonal antibody (28622-1-AP)

    WB analysis using 28622-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28622-1-AP (BCAT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BCAT1 Polyclonal antibody (28622-1-AP)

    IHC staining of human liver cancer using 28622-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28622-1-AP (BCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BCAT1 Polyclonal antibody (28622-1-AP)

    IHC staining of human lung cancer using 28622-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28622-1-AP (BCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using BCAT1 Polyclonal antibody (28622-1-AP)

    IHC staining of human gliomas using 28622-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 28622-1-AP (BCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inC6 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28622-1-AP targets BCAT1 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCAT1 fusion protein Ag28998
Full Name branched chain aminotransferase 1, cytosolic
Calculated Molecular Weight 320 aa, 36 kDa
Observed Molecular Weight 43-48 kDa
GenBank Accession NumberBC033864
Gene Symbol BCAT1
Gene ID (NCBI) 586
RRIDAB_2881183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).

Protocols

Product Specific Protocols
WB protocol for BCAT1 antibody 28622-1-APDownload protocol
IHC protocol for BCAT1 antibody 28622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncol Rep

BCAT1 overexpression regulates proliferation and c‑Myc/GLUT1 signaling in head and neck squamous cell carcinoma.

Authors - Hongming Wang
View Article
mouseWB,IHC

Biochim Biophys Acta Mol Basis Dis

Targeted inhibition of branched-chain amino acid metabolism drives apoptosis of glioblastoma by facilitating ubiquitin degradation of Mfn2 and oxidative stress

Authors - Zhuo Lu
View Article