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LPL Polyclonal antibody

LPL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28602-1-AP

Synonyms

HDLCQ11, LIPD, lipoprotein lipase, LPL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LPL Polyclonal antibody (28602-1-AP)

    WB analysis using 28602-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 28602-1-AP (LPL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using LPL Polyclonal antibody (28602-1-AP)

    IHC staining of mouse brown adipose using 28602-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 28602-1-AP (LPL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inmouse brown adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28602-1-AP targets LPL in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein
Full Name lipoprotein lipase
Calculated Molecular Weight 475 aa, 53 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC011353
Gene Symbol LPL
Gene ID (NCBI) 4023
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lipoprotein lipase (LPL) is a key enzyme in lipid and lipoprotein metabolism. The canonical role of LPL involves the hydrolysis of triglyceride-rich lipoproteins for the provision of FFAs to metabolic tissues. LPL is highly expressed in adipocytes and myocytes as well as macrophages. Its protein level and activity are tightly regulated by multiple mechanisms in response to the metabolic state and energy demands of the cell . (PMID: 33277156, PMID: 33172164)

Protocols

Product Specific Protocols
WB protocol for LPL antibody 28602-1-APDownload protocol
IHC protocol for LPL antibody 28602-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Cancers (Basel)

Triptolide Reduces Neoplastic Progression in Hepatocellular Carcinoma by Downregulating the Lipid Lipase Signaling Pathway

Authors - Wei Chang
View Article