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HCFC1 Polyclonal antibody

HCFC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 28569-1-AP

Synonyms

C1 factor, CFF, HCF, HCF 1, HCF1, HCFC1, HFC1, Host cell factor 1, VCAF, VP16 accessory protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HCFC1 Polyclonal antibody (28569-1-AP)

    WB analysis using 28569-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28569-1-AP (HCFC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using HCFC1 Polyclonal antibody (28569-1-AP)

    IP experiment of HeLa using 28569-1-AP

    IP result of anti-HCFC1 (IP:28569-1-AP, 4ug; Detection:28569-1-AP 1:20000) with HeLa cells lysate 1600 ug.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using HCFC1 Polyclonal antibody (28569-1-AP)

    IHC staining of mouse skin using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using HCFC1 Polyclonal antibody (28569-1-AP)

    IHC staining of mouse skin using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HCFC1 Polyclonal antibody (28569-1-AP)

    IHC staining of human lung cancer using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HCFC1 Polyclonal antibody (28569-1-AP)

    IHC staining of human lung cancer using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using HCFC1 Polyclonal antibody (28569-1-AP)

    IHC staining of human urothelial carcinoma using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using HCFC1 Polyclonal antibody (28569-1-AP)

    IHC staining of human urothelial carcinoma using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using HCFC1 Polyclonal antibody (28569-1-AP)

    IHC staining of mouse cerebellum using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using HCFC1 Polyclonal antibody (28569-1-AP)

    IHC staining of mouse cerebellum using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using HCFC1 Polyclonal antibody (28569-1-AP)

    IHC staining of mouse lung using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using HCFC1 Polyclonal antibody (28569-1-AP)

    IHC staining of mouse lung using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using HCFC1 Polyclonal antibody (28569-1-AP)

    IHC staining of rat cerebellum using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using HCFC1 Polyclonal antibody (28569-1-AP)

    IHC staining of rat cerebellum using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using HCFC1 Polyclonal antibody (28569-1-AP)

    IHC staining of rat lung using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using HCFC1 Polyclonal antibody (28569-1-AP)

    IHC staining of rat lung using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skin tissue using HCFC1 Polyclonal antibody (28569-1-AP)

    IHC staining of rat skin using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skin tissue using HCFC1 Polyclonal antibody (28569-1-AP)

    IHC staining of rat skin using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HCFC1 Polyclonal antibody (28569-1-AP)

    IF Staining of HeLa using 28569-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HCFC1 antibody (28569-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, NIH/3T3 cells, PC-12 cells, C2C12 cells, C6 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse skin tissue, human lung cancer tissue, human urothelial carcinoma tissue, mouse cerebellum tissue, mouse lung tissue, rat cerebellum tissue, rat lung tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28569-1-AP targets HCFC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HCFC1 fusion protein Ag29663
Full Name host cell factor C1 (VP16-accessory protein)
Calculated Molecular Weight 209 kDa
Observed Molecular Weight300 kDa, 100-150 kDa
GenBank Accession NumberNM_005334
Gene Symbol HCFC1
Gene ID (NCBI) 3054
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HCFC1 (also known as HCF-1) was discovered in studies of herpes simplex virus (HSV) transcription. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in the control of the cell cycle and transcriptional regulation during herpes simplex virus infection. HCFC1 is a large protein of ∼300 kDa and is proteolytically processed into N- and C-terminal fragments of ∼150 kDa each.(PMID: 21295698 )

Protocols

Product Specific Protocols
WB protocol for HCFC1 antibody 28569-1-APDownload protocol
IHC protocol for HCFC1 antibody 28569-1-APDownload protocol
IF protocol for HCFC1 antibody 28569-1-APDownload protocol
IP protocol for HCFC1 antibody 28569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols