F4/80 Polyclonal antibody
F4/80 Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, rat and More (2)
Applications
WB, IHC, IF/ICC, IF-P, ELISA
Conjugate
Unconjugated
219
Cat no : 28463-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse spleen tissue, rat spleen tissue, rat liver tissue, mouse lung tissue, mouse heart tissue, mouse brain tissue, mouse liver tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue, mouse liver tissue |
| Positive IF/ICC detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 15 publications below |
| IHC | See 108 publications below |
| IF | See 92 publications below |
| FC | See 8 publications below |
Product Information
28463-1-AP targets F4/80 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | F4/80 fusion protein Ag28252 |
| Full Name | EGF-like module containing, mucin-like, hormone receptor-like sequence 1 |
| Calculated Molecular Weight | 102 kDa |
| GenBank Accession Number | NM_010130 |
| Gene Symbol | F4/80 |
| Gene ID (NCBI) | 13733 |
| RRID | AB_2881149 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for F4/80 antibody 28463-1-AP | Download protocol |
| IF protocol for F4/80 antibody 28463-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioact Mater Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis | ||
Nat Commun Dubosiella newyorkensis modulates immune tolerance in colitis via the L-lysine-activated AhR-IDO1-Kyn pathway | ||
Redox Biol Cathepsin C from extracellular histone-induced M1 alveolar macrophages promotes NETosis during lung ischemia-reperfusion injury | ||
Biomaterials Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia. | ||
Biomaterials A myogenic niche with a proper mechanical stress environment improves abdominal wall muscle repair by modulating immunity and preventing fibrosis. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kannan (Verified Customer) (06-13-2023) | AR using EDTApH9. overnight incubation of primary antibody at 4c.
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