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TH1L Polyclonal antibody

TH1L Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28417-1-AP

Synonyms

HSPC130, Negative elongation factor C/D, NELF C, NELF C/D, NELF D, NELFD, TH1, TH1 like (Drosophila), TH1 like protein, TH1L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TH1L Polyclonal antibody (28417-1-AP)

    WB analysis using 28417-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28417-1-AP (TH1L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TH1L Polyclonal antibody (28417-1-AP)

    IF Staining of HeLa using 28417-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 28417-1-AP (TH1L antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28417-1-AP targets TH1L in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TH1L fusion protein Ag29262
Full Name TH1-like (Drosophila)
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC014952
Gene Symbol TH1L
Gene ID (NCBI) 51497
RRIDAB_2881137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TH1L antibody 28417-1-APDownload protocol
IF protocol for TH1L antibody 28417-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols