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LYVE1 Polyclonal antibody

LYVE1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28321-1-AP

Synonyms

HAR, Extracellular link domain-containing protein 1, CRSBP-1, CRSBP1, CRSBP 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LYVE1 Polyclonal antibody (28321-1-AP)

    WB analysis using 28321-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28321-1-AP (LYVE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LYVE1 Polyclonal antibody (28321-1-AP)

    WB analysis using 28321-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28321-1-AP (LYVE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat liver tissue using LYVE1 Polyclonal antibody (28321-1-AP)

    IHC staining of rat liver using 28321-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28321-1-AP (LYVE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using LYVE1 Polyclonal antibody (28321-1-AP)

    IHC staining of rat liver using 28321-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28321-1-AP (LYVE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHUVEC cells, bEnd.3 cells, mouse heart tissue, rat heart tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28321-1-AP targets LYVE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LYVE1 fusion protein Ag28131
Full Name lymphatic vessel endothelial hyaluronan receptor 1
Calculated Molecular Weight 322 aa, 35 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC026231
Gene Symbol LYVE1
Gene ID (NCBI) 10894
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LYVE1 is a 322-amino acid type-I integral membrane glycoprotein primarily expressed on lymphatic endothelial cells. LYVE1 is a CD44 homologue. It acts as a receptor and binds to both soluble and immobilized hyaluronan. LYVE1 may function in lymphatic hyaluronan transport and have a role in tumor metastasis.

Protocols

Product Specific Protocols
WB protocol for LYVE1 antibody 28321-1-APDownload protocol
IHC protocol for LYVE1 antibody 28321-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols