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ABCB4 Polyclonal antibody

ABCB4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27726-1-AP

Synonyms

ABC21, ABCB4, GBD1, MDR2, MDR2/3, MDR3, Multidrug resistance protein 3, P glycoprotein 3, PFIC 3, PGY3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABCB4 Polyclonal antibody (27726-1-AP)

    WB analysis using 27726-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27726-1-AP (ABCB4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27726-1-AP targets ABCB4 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCB4 fusion protein Ag26840
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 4
Calculated Molecular Weight 1286 aa, 142 kDa
Observed Molecular Weight130-140 kDa
GenBank Accession NumberBC042531
Gene Symbol ABCB4
Gene ID (NCBI) 5244
RRIDAB_2918128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ABCB4 antibody 27726-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols