Tested Applications
| Positive WB detected in | Caco-2 cells, mouse kidney tissue, PC-3 cells, mouse small intestine tissue |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27575-1-AP targets SLC6A19 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24362 Product name: Recombinant human SLC6A19 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 295-403 aa of BC148801 Sequence: VHNNCEKDSVIVSIINGFTSVYVAIVVYSVIGFRATQRYDDCFSTNILTLINGFDLPEGNVTQENFVDMQQRCNASDPAAYAQLVFQTCDINAFLSEAVEGTGLAFIVF Predict reactive species |
| Full Name | solute carrier family 6 (neutral amino acid transporter), member 19 |
| Calculated Molecular Weight | 634 aa, 71 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC148801 |
| Gene Symbol | SLC6A19 |
| Gene ID (NCBI) | 340024 |
| RRID | AB_3085970 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q695T7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC6A19, also named as B0AT1, encodes system B(0) transmembrane protein that mediates epithelial resorption of neutral amino acids across the apical membrane in kidney and intestine (PubMed: 15044460). The protein contains 634 amino acids and has 12 predicted transmembrane regions with highly expression in human kidney and small intestine and minimal in pancreas (PubMed: 15286787). The expression of SLC6A19 in intestinal cells depends on angiotensin converting enzyme 2 which is the cellular receptor for SARS-CoV-2 (PubMed: 32132184). Mutations in SLC6A19 cause Hartnup disease (PMID: 15286787, PMID: 15286788).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC6A19 antibody 27575-1-AP | Download protocol |
| WB protocol for SLC6A19 antibody 27575-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
