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PSMD8 Polyclonal antibody

PSMD8 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27504-1-AP

Synonyms

HIP6, HYPF, Nin1p, p31, PSMD8, Rpn12, S14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMD8 Polyclonal antibody (27504-1-AP)

    WB analysis using 27504-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27504-1-AP (PSMD8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PSMD8 Polyclonal antibody (27504-1-AP)

    IF Staining of U2OS using 27504-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PSMD8 antibody (27504-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27504-1-AP targets PSMD8 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMD8 fusion protein Ag26567
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 8
Calculated Molecular Weight 257 aa, 30 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC001164
Gene Symbol PSMD8
Gene ID (NCBI) 5714
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Proteasome 26S Subunit, Non-ATPase (PSMD) gene family, which includes the PSMD1 to PSMD14, regulate ubiqutinated protein breakdown in circulation as well as tumorigenesis. Studies have shown that PSMD8 interacts with the sperm adhesin AQN1 to limit polyfertilization. PSMD8 was more expressed in normal skeletal muscle, cardiac muscle, and tongue muscle; among malignant tumors, PSMD8 was expressed in ovarian cancer, testicular cancer, glioma, and melanoma(PMID:37349676).

Protocols

Product Specific Protocols
WB protocol for PSMD8 antibody 27504-1-APDownload protocol
IF protocol for PSMD8 antibody 27504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols