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UPK1B Polyclonal antibody

UPK1B Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 26853-1-AP

Synonyms

Tetraspanin 20, Tspan 20, TSPAN20, UP1b, UPIB, UPK1, UPK1B, uroplakin 1B, Uroplakin Ib

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using UPK1B Polyclonal antibody (26853-1-AP)

    IHC staining of human urothelial carcinoma using 26853-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 26853-1-AP (UPK1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using UPK1B Polyclonal antibody (26853-1-AP)

    IHC staining of human urothelial carcinoma using 26853-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 26853-1-AP (UPK1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using UPK1B Polyclonal antibody (26853-1-AP)

    IHC staining of mouse bladder using 26853-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 26853-1-AP (UPK1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using UPK1B Polyclonal antibody (26853-1-AP)

    IHC staining of mouse bladder using 26853-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 26853-1-AP (UPK1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman urothelial carcinoma tissue, mouse bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26853-1-AP targets UPK1B in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UPK1B fusion protein Ag25471
Full Name uroplakin 1B
GenBank Accession NumberBC063568
Gene Symbol UPK1B
Gene ID (NCBI) 7348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-1B (UPK1B) belongs to the tetraspanin (TM4SF) family. UPK1B can promote the proliferation, invasion and metastasis of tumor cells and regulate the development and progression of tumors (PMID: 30229818).

Protocols

Product Specific Protocols
IHC protocol for UPK1B antibody 26853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols