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  • Featured Product
  • KD/KO Validated

ZC3H11A Polyclonal antibody

ZC3H11A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26081-1-AP

Synonyms

KIAA0663, ZC3H11A, ZC3HDC11A

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse ovary tissue using ZC3H11A Polyclonal antibody (26081-1-AP)

    WB analysis of mouse ovary using 26081-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 26081-1-AP (ZC3H11A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ZC3H11A Polyclonal antibody (26081-1-AP)

    WB analysis of HeLa using 26081-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26081-1-AP (ZC3H11A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ZC3H11A Polyclonal antibody (26081-1-AP)

    IF Staining of HEK-293 using 26081-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ZC3H11A antibody (26081-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ZC3H11A Polyclonal antibody (26081-1-AP)

    IF Staining of HEK-293 using 26081-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ZC3H11A antibody (26081-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse ovary tissue, HeLa cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26081-1-AP targets ZC3H11A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitypig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZC3H11A fusion protein Ag23424
Full Name zinc finger CCCH-type containing 11A
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC014268
Gene Symbol ZC3H11A
Gene ID (NCBI) 9877
RRIDAB_2880368
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZC3H11A is a RNA-binding protein that interacts with purine-rich sequences and is involved in nuclear mRNA export.

Protocols

Product Specific Protocols
WB protocol for ZC3H11A antibody 26081-1-APDownload protocol
IF protocol for ZC3H11A antibody 26081-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

ACS Infect Dis

Porcine ZC3H11A Is Essential for the Proliferation of Pseudorabies Virus and Porcine Circovirus 2.

Authors - Lin Yang
  • KO Validated
View Article