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Conjugate
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Conjugate
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Conjugate
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FMN1 Polyclonal antibody

FMN1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25982-1-AP

Synonyms

FMN, FMN1, FMN1 Ens, formin 1, LD

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A549 cells using FMN1 Polyclonal antibody (25982-1-AP)

    WB analysis of A549 using 25982-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25982-1-AP (FMN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FMN1 Polyclonal antibody (25982-1-AP)

    IF Staining of A549 using 25982-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 25982-1-AP (FMN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25982-1-AP is FMN1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FMN1 fusion protein Ag23059
Full Name formin 1
Calculated Molecular Weight 1196 aa, 132 kDa
Observed Molecular Weight 160 kDa
GenBank Accession NumberBC103692
Gene Symbol FMN1
Gene ID (NCBI) 342184
RRIDAB_2880319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FMN1 antibody 25982-1-APDownload protocol
IF protocol for FMN1 antibody 25982-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Molecules

MOBT Alleviates Pulmonary Fibrosis through an lncITPF-hnRNP-l-Complex-Mediated Signaling Pathway

Authors - Pan Xu
View Article