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- KD/KO Validated
CD63 Polyclonal antibody
CD63 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (6)
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA
Conjugate
Unconjugated
369
Cat no : 25682-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human urine exosomes tissue, human serum exosomes |
| Positive IHC detected in | human malignant melanoma tissue, human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human malignant melanoma tissue |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 343 publications below |
| IHC | See 7 publications below |
| IF | See 17 publications below |
| IP | See 2 publications below |
| FC | See 2 publications below |
Product Information
25682-1-AP targets CD63 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig, rabbit, canine, monkey, chicken, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD63 fusion protein Ag19690 |
| Full Name | CD63 molecule |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 28-35 kDa |
| GenBank Accession Number | BC002349 |
| Gene Symbol | CD63 |
| Gene ID (NCBI) | 967 |
| RRID | AB_2783831 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD63 antibody 25682-1-AP | Download protocol |
| IHC protocol for CD63 antibody 25682-1-AP | Download protocol |
| IF protocol for CD63 antibody 25682-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Nano Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration. | ||
Adv Sci (Weinh) Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury | ||
J Extracell Vesicles Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours. | ||
Biosens Bioelectron Aptamer-bivalent-cholesterol-mediated proximity entropy-driven exosomal protein reporter for tumor diagnosis | ||
Small Methods Stimuli-Mediated Specific Isolation of Exosomes from Blood Plasma for High-Throughput Profiling of Cancer Biomarkers. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Federica (Verified Customer) (11-30-2023) | I used a Leica Bond Max on a healthy Tonsil tissue. Antigen Retrival ER2 95C for 30min
 |
FH Nydia (Verified Customer) (06-11-2022) | The HeLa cells as well as Lamp1-expressing stem cells used in the immunostaining were transfected or alternatively transfected by splitting the cells later on the glass coverslips. In order to increase cell spreading and adhesion, the coverslips used were acid washed and treated with fibronectin. Afterwards, the cells were fixed with 4% PFA, permeabilized with 0.2% Triton X-100 in phosphate- buffered saline (PBS) for 10 minutes and for 1 hour the cells were blocked with 5% BSA in PBS. Then overnight, at 4 degrees Celsius, the primary antibodies were added followed by a wash with PBS 3 times.
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FH Guorong (Verified Customer) (03-22-2022) | Smears between 30 and 60 kDa was detected
 |
FH David (Verified Customer) (03-30-2020) | Used for both SH-SY5Y lysate and exosomes/ extracellular vesicles. Very good signal in lysates (could be used at much higher dilution). Also good signal in exosomes, which are notoriously difficult to assess by immunoblot. Very impressive results compared to other markers.
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