• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

PKM Polyclonal antibody

PKM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 25659-1-AP

Synonyms

PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM2, PK-M2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of KEK293 cells using PKM Polyclonal antibody (25659-1-AP)

    WB analysis of KEK293 cells using 25659-1-AP

    WB result of PKM antibody (25659-1-AP, 1:3000) with si-control and si-PKM transfected HEK293 cell.

  • Western Blot (WB) analysis of various lysates using PKM Polyclonal antibody (25659-1-AP)

    WB analysis using 25659-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PKM Polyclonal antibody (25659-1-AP)

    WB analysis using 25659-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PKM Polyclonal antibody (25659-1-AP)

    WB analysis of HEK-293 using 25659-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PKM Polyclonal antibody (25659-1-AP)

    WB analysis of MCF-7 using 25659-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using PKM Polyclonal antibody (25659-1-AP)

    WB analysis of COLO 320 using 25659-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using PKM Polyclonal antibody (25659-1-AP)

    WB analysis of SMMC-7721 using 25659-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PKM Polyclonal antibody (25659-1-AP)

    IHC staining of human liver cancer using 25659-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25659-1-AP (PKM Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PKM Polyclonal antibody (25659-1-AP)

    IHC staining of human liver cancer using 25659-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25659-1-AP (PKM Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human normal colon using PKM Polyclonal antibody (25659-1-AP)

    IHC staining of human normal colon using 25659-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25659-1-AP (PKM antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKM Polyclonal antibody (25659-1-AP)

    IF Staining of HeLa using 25659-1-AP

    Immunofluorescent analysis of HeLa cells using 25659-1-AP (PKM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKM Polyclonal antibody (25659-1-AP)

    IF Staining of HeLa using 25659-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKM antibody (25659-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, COLO 320 cells, SMMC-7721 cells, KEK293 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, NIH/3T3 cells, mouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IHC detected inhuman liver cancer tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25659-1-AP targets PKM in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKM fusion protein Ag20270
Full Name PKM pyruvate kinase, muscle
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC000481
Gene Symbol PKM
Gene ID (NCBI) 5315
RRIDAB_2880181
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKM, also named as OIP3, PK2, PK3, PK-M1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2.(PMID:18337823). This antibody can recognize both PK-M1 and PK-M2.

Protocols

Product Specific Protocols
WB protocol for PKM antibody 25659-1-APDownload protocol
IHC protocol for PKM antibody 25659-1-APDownload protocol
IF protocol for PKM antibody 25659-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

EMBO Rep

Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.

Authors - Guang Yang
View Article
humanWB

Oncogene

HMGB1/SET/HAT1 complex-mediated SASH1 repression drives glycolysis and metastasis in lung adenocarcinoma

Authors - Fan Kou
View Article
humanWB,IP,CoIP

Int J Mol Sci

Acetyltransferase P300 Regulates Glucose Metabolic Reprogramming through Catalyzing Succinylation in Lung Cancer

Authors - Qingzhi Ma
View Article
humanWB

Cell Mol Biol Lett

N6-methyladenosine-modified SRPK1 promotes aerobic glycolysis of lung adenocarcinoma via PKM splicing

Authors - Anqi Wang
View Article