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APP/Beta Amyloid Polyclonal antibody

Name: APP/Beta Amyloid Polyclonal antibody
Brand: Proteintech
SKU: 25524-1-AP
Rating: 4.50 (2 reviews)

APP/Beta Amyloid Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Reviews (2)

Cat no : 25524-1-AP

Synonyms

amyloid precursor protein, APP, Alzheimer disease amyloid protein, Alzheimer disease amyloid A4 protein homolog, AD1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 25524-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HAP1 using 25524-1-AP
HAP1 (WT and APP KO) lysates prepared with RIPA buffer, 50μg protein loaded. 25524-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
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HAP1 (WT and APP KO) lysates prepared with RIPA buffer, 50μg protein loaded. 25524-1-AP incubated at 1:500 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
WB analysis using 25524-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP/Beta Amyloid antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 25524-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 25524-1-AP (beta Amyloid (C-Terminal) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of 3T3-L1 using 25524-1-AP
3T3-L1 cells were subjected to SDS PAGE followed by western blot with 25524-1-AP (APP, C-Terminal antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
$HAP1 lysates prepared and IP of APP performed using 2.0 μg of 25524-1-AP coupled to protein A- Dynabeads beads. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate.These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.$
IP experiment of HAP1 using 25524-1-AP
HAP1 lysates prepared and IP of APP performed using 2.0 μg of 25524-1-AP coupled to protein A- Dynabeads beads. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate.These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
×
HAP1 lysates prepared and IP of APP performed using 2.0 μg of 25524-1-AP coupled to protein A- Dynabeads beads. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate.These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
IHC staining of human gliomas using 25524-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 25524-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 25524-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 10x lens).
IHC staining of human brain using 25524-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25524-1-AP (APP, C-Terminal antibody at dilution of 1:200 (under 40x lens).
IF Staining of SH-SY5Y using 25524-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using APP/Beta Amyloid antibody (25524-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 25524-1-AP

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Tested Applications

Positive WB detected in	SH-SY5Y cells, HAP1, mouse brain tissue, HeLa cells, rat brain tissue, C6 cells
Positive IHC detected in	human gliomas tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 58 publications below
IHC	See 17 publications below
IF	See 18 publications below

Product Information

25524-1-AP targets APP/Beta Amyloid in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	APP/Beta Amyloid fusion protein Ag22408
Full Name	amyloid beta (A4) precursor protein
Observed Molecular Weight	100 kDa
GenBank Accession Number	BC065529
Gene Symbol	APP
Gene ID (NCBI)	351
RRID	AB_2880118
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. This antibody can recognize several isoforms of both mature and immature amyloid beta (A4) precursor protein, including APP770, APP677, APP695, APP696, APP733, APP751, APP752, and APP639. APP can be cleaved into several chains, this antibody could recognize fragments C99, Amyloid-beta protein 42, Amyloid-beta protein 40, C83, P3(40), C80, Gamma-secretase C-terminal fragment 59, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 50, C31.

Protocols

Product Specific Protocols
WB protocol for APP/Beta Amyloid antibody 25524-1-AP	Download protocol
IHC protocol for APP/Beta Amyloid antibody 25524-1-AP	Download protocol
IF protocol for APP/Beta Amyloid antibody 25524-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Clin Invest Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer Authors - Xiaochao Tan View Article
mouse	IF	Neuron Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2. Authors - Nathalie Escande-Beillard View Article
human	WB	Small Brain-Penetration and Neuron-Targeting DNA Nanoflowers Co-Delivering miR-124 and Rutin for Synergistic Therapy of Alzheimer's Disease. Authors - Qin Ouyang View Article
rat	IHC	Biomater Res Controlled release of Clenbuterol from a hydroxyapatite carrier for the treatment of Alzheimer's Disease Authors - Yi-Wen Lin View Article
mouse	IHC	Redox Biol Cognitive enhancement and neuroprotective effects of OABL, a sesquiterpene lactone in 5xFAD Alzheimer's disease mice model. Authors - Jiang-Jiang Tang View Article
mouse,rat	WB,IF	Free Radic Biol Med The neuroprotective effects of phosphocreatine on amyloid beta 25-35-induced differentiated neuronal cell death through inhibition of AKT/GSK-3β /Tau/APP/CDK5 pathways in vivo and vitro Authors - Jie Ai View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Jorge (Verified Customer) (03-28-2023)

Test to evaluate the efficacy of the antibody. Tested on SH-SY5Y APPswe cells differentiated with 10 uM retic acid for 5 days. DAPT treatment 10 uM for 24h. Cells lysed with RIPA buffer. Loading different protein concentrations (ug). Tricine SDS-PAGE 16% acrylamide gel. Transfered on PVDF membrane. Blocking 5% milk. Dilution 1:4000 in TBS-Tw 0.05%.

Applications: Western Blot
Primary Antibody Dilution: 1:4000
Cell Tissue Type: Cell line SHSY5Y APPswe

APP/Beta Amyloid Antibody Western Blot validation (1:4000 dilution) in Cell line SHSY5Y APPswe (Cat no:25524-1-AP)

JIMMY (Verified Customer) (04-15-2019)

This antibody detects APP in the brain of 12 months C57BL/6 mice.

Applications: Western Blot,

APP/Beta Amyloid Polyclonal antibody

APP/Beta Amyloid Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 25524-1-AP

WB analysis of HAP1 using 25524-1-AP

WB analysis using 25524-1-AP

WB analysis of mouse brain using 25524-1-AP

WB analysis of 3T3-L1 using 25524-1-AP

IP experiment of HAP1 using 25524-1-AP

IHC staining of human gliomas using 25524-1-AP

IHC staining of human gliomas using 25524-1-AP

IHC staining of human brain using 25524-1-AP

IHC staining of human brain using 25524-1-AP

IF Staining of SH-SY5Y using 25524-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer

Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.

Brain-Penetration and Neuron-Targeting DNA Nanoflowers Co-Delivering miR-124 and Rutin for Synergistic Therapy of Alzheimer's Disease.

Controlled release of Clenbuterol from a hydroxyapatite carrier for the treatment of Alzheimer's Disease

Cognitive enhancement and neuroprotective effects of OABL, a sesquiterpene lactone in 5xFAD Alzheimer's disease mice model.

The neuroprotective effects of phosphocreatine on amyloid beta 25-35-induced differentiated neuronal cell death through inhibition of AKT/GSK-3β /Tau/APP/CDK5 pathways in vivo and vitro

Reviews