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GRAMD1A Polyclonal antibody

GRAMD1A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25487-1-AP

Synonyms

GRAM domain containing 1A, GRAMD1A, KIAA1533

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRAMD1A Polyclonal antibody (25487-1-AP)

    WB analysis using 25487-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25487-1-AP (GRAMD1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells, HEK-293T cells, HeLa cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25487-1-AP targets GRAMD1A in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRAMD1A fusion protein Ag22221
Full Name GRAM domain containing 1A
Calculated Molecular Weight 724 aa, 81 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC014077
Gene Symbol GRAMD1A
Gene ID (NCBI) 57655
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein Aster-A (GRAMD1A) is a cholesterol transporter, which contains unique domains for binding cholesterol and the plasma membrane (PM), thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the endoplasmic reticulum (ER). It was reported that plays a role in autophagy regulation and is required for the biogenesis of the autophagosome (PMID:31222192). It can be detected as 80 kDa.

Protocols

Product Specific Protocols
WB protocol for GRAMD1A antibody 25487-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols