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TBX15 Polyclonal antibody

TBX15 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25148-1-AP

Synonyms

T box 15, TBX14, TBX15

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBX15 Polyclonal antibody (25148-1-AP)

    WB analysis using 25148-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25148-1-AP (TBX15 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25148-1-AP is TBX15 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBX15 fusion protein Ag18423
Full Name T-box 15
Calculated Molecular Weight 602 aa, 66 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC122553
Gene Symbol TBX15
Gene ID (NCBI) 6913
RRIDAB_2879924
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBX15 is a transcription factor from the T-box family and is a highly pleiotropic gene expressed in multiple tissues at different stages of development. It is required for skeletal development and deletions in this gene cause severe skeletal malformation. In addition, TBX15 also plays a role in the differentiation of brown and beige adipocytes (PMID: 28007980). The predicted molecular weight of TBX15 is 66 kDa. With modification, the MW of TBX15 will be migrated to 50 kDa.

Protocols

Product Specific Protocols
WB protocol for TBX15 antibody 25148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

iScience

TBX15 facilitates malignant progression of glioma by transcriptional activation of TXDNC5

Authors - Yuyuan Ge
View Article