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UHRF1BP1L Polyclonal antibody

UHRF1BP1L Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25121-1-AP

Synonyms

KIAA0701, UHRF1 binding protein 1 like, UHRF1BP1L

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using UHRF1BP1L Polyclonal antibody (25121-1-AP)

    WB analysis of mouse brain using 25121-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25121-1-AP (UHRF1BP1L Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using UHRF1BP1L Polyclonal antibody (25121-1-AP)

    IP experiment of mouse brain using 25121-1-AP

    IP result of anti-UHRF1BP1L (IP:25121-1-AP, 5ug; Detection:25121-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25121-1-AP is UHRF1BP1L Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UHRF1BP1L fusion protein Ag19141
Full Name UHRF1 binding protein 1-like
Calculated Molecular Weight 1464 aa, 164 kDa
Observed Molecular Weight 160-165 kDa
GenBank Accession NumberBC127017
Gene Symbol UHRF1BP1L
Gene ID (NCBI) 23074
RRIDAB_2879909
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UHRF1BP1L antibody 25121-1-APDownload protocol
IP protocol for UHRF1BP1L antibody 25121-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Daniel (Verified Customer) (10-26-2022)

This antibody works well and produced little background. Works best in PVDF membranes.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa, Mt4 cells
UHRF1BP1L Antibody Western Blot validation (1:1000 dilution) in HeLa, Mt4 cells (Cat no:25121-1-AP)