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hCG Alpha Polyclonal antibody

hCG Alpha Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25014-1-AP

Synonyms

CG ALPHA, CGA, Choriogonadotropin alpha chain, Follitropin alpha chain, FSH alpha, FSHA, GPHa, GPHA1, HCG, hCG alpha, LHA, LSH alpha, Lutropin alpha chain, Thyrotropin alpha chain, TSH alpha, TSHA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using hCG Alpha Polyclonal antibody (25014-1-AP)

    WB analysis using 25014-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25014-1-AP (hCG alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using hCG Alpha Polyclonal antibody (25014-1-AP)

    WB analysis of human placenta using 25014-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 25014-1-AP (hCG alpha antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using hCG Alpha Polyclonal antibody (25014-1-AP)

    IHC staining of human placenta using 25014-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25014-1-AP (hCG Alpha antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using hCG Alpha Polyclonal antibody (25014-1-AP)

    IHC staining of human placenta using 25014-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25014-1-AP (hCG Alpha antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJAR cells, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25014-1-AP targets hCG Alpha in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen hCG Alpha fusion protein Ag17313
Full Name glycoprotein hormones, alpha polypeptide
Calculated Molecular Weight 116 aa, 13 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC020782
Gene Symbol hCG Alpha
Gene ID (NCBI) 1081
RRIDAB_2879846
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for hCG Alpha antibody 25014-1-APDownload protocol
IHC protocol for hCG Alpha antibody 25014-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Immunother Cancer

TSH-TSHR axis promotes tumor immune evasion.

Authors - Zhenghao Wu
View Article
humanIHC

J Clin Endocrinol Metab

A Patient with a Thyrotropin-secreting Microadenoma and Resistance to Thyroid Hormone (P453T).

Authors - Xiaochun Teng
View Article
humanWB

iScience

L3MBTL2-mediated CGA transcriptional suppression promotes pancreatic cancer progression through modulating autophagy.

Authors - Hua Huang
View Article
humanIHC

Front Endocrinol (Lausanne)

Four novel mutations identification in 17 beta-hydroxysteroid dehydrogenase-3 deficiency and our clinical experience: possible benefits of early treatment

Authors - Yunpeng Wang
View Article