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  • Featured Product
  • KD/KO Validated

KIAA0182 Polyclonal antibody

KIAA0182 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24947-1-AP

Synonyms

Genetic suppressor element 1, GSE1, KIAA0182

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA0182 Polyclonal antibody (24947-1-AP)

    WB analysis using 24947-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24947-1-AP (KIAA0182 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KIAA0182 Polyclonal antibody (24947-1-AP)

    IHC staining of mouse testis using 24947-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using KIAA0182 Polyclonal antibody (24947-1-AP)

    IHC staining of human kidney using 24947-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using KIAA0182 Polyclonal antibody (24947-1-AP)

    IHC staining of human kidney using 24947-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KIAA0182 Polyclonal antibody (24947-1-AP)

    IHC staining of mouse testis using 24947-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using KIAA0182 Polyclonal antibody (24947-1-AP)

    IF Staining of U2OS using 24947-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KIAA0182 antibody (24947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inmouse testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24947-1-AP targets KIAA0182 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA0182 fusion protein Ag20674
Full Name KIAA0182
Calculated Molecular Weight 1217 aa, 136 kDa
Observed Molecular Weight 136 kDa
GenBank Accession NumberBC037556
Gene Symbol KIAA0182
Gene ID (NCBI) 23199
RRIDAB_2879815
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KIAA0182 antibody 24947-1-APDownload protocol
IHC protocol for KIAA0182 antibody 24947-1-APDownload protocol
IF protocol for KIAA0182 antibody 24947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell Stem Cell

Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis.

Authors - Miller Huang
  • KO Validated
View Article
humanWB

Nat Commun

The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs

Authors - Andreas Herchenröther
View Article
humanWB

Sci Adv

Targeting the scaffolding role of LSD1 (KDM1A) poises acute myeloid leukemia cells for retinoic acid-induced differentiation.

Authors - Roberto Ravasio
View Article
humanWB

EMBO Mol Med

LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma.

Authors - Lukas Leiendecker
View Article
humanWB,IHC

J Biol Chem

GSE1 predicts poor survival outcome in gastric cancer patients by SLC7A5 enhancement of tumor growth and metastasis.

Authors - Keshuo Ding
  • KD Validated
View Article
humanWB

Biochem Biophys Res Commun

GSE1 negative regulation by miR-489-5p promotes breast cancer cell proliferation and invasion.

Authors - Peng Chai
View Article