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FPGT Polyclonal antibody

FPGT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24937-1-AP

Synonyms

FPGT, GDP L fucose diphosphorylase, GDP L fucose pyrophosphorylase, GFPP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using FPGT Polyclonal antibody (24937-1-AP)

    WB analysis of mouse colon using 24937-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 24937-1-AP (FPGT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using FPGT Polyclonal antibody (24937-1-AP)

    IHC staining of human colon using 24937-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24937-1-AP (FPGT Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using FPGT Polyclonal antibody (24937-1-AP)

    IHC staining of human colon using 24937-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24937-1-AP (FPGT Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using FPGT Polyclonal antibody (24937-1-AP)

    IHC staining of human kidney using 24937-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24937-1-AP (FPGT Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using FPGT Polyclonal antibody (24937-1-AP)

    IHC staining of human kidney using 24937-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24937-1-AP (FPGT Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse colon tissue
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24937-1-AP is FPGT Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FPGT fusion protein Ag20655
Full Name fucose-1-phosphate guanylyltransferase
Calculated Molecular Weight 67 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC032308
Gene Symbol FPGT
Gene ID (NCBI) 8790
RRIDAB_2879808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FPGT(Fucose-1-phosphate guanylyltransferase) is also named as GFPP. This protein catalyzes the formation of GDP-L-fucose from GTP and L-fucose-1-phosphate. It also functions as a salvage pathway to reutilize L-fucose arising from the turnover of glycoproteins and glycolipids. FPGT has 6 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for FPGT antibody 24937-1-APDownload protocol
IHC protocol for FPGT antibody 24937-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols