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C1RL Polyclonal antibody

C1RL Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24820-1-AP

Synonyms

C1r like protein, C1r LP, C1RL, C1RL1, C1RLP, CLSPa

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C1RL Polyclonal antibody (24820-1-AP)

    WB analysis using 24820-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24820-1-AP (C1RL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using C1RL Polyclonal antibody (24820-1-AP)

    IF Staining of U2OS using 24820-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using C1RL antibody (24820-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman plasma
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24820-1-AP targets C1RL in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1RL fusion protein Ag20324
Full Name complement component 1, r subcomponent-like
Calculated Molecular Weight 487 aa, 53 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC156780
Gene Symbol C1RL
Gene ID (NCBI) 51279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C1r-like serine protease analog protein (CLSPa), also named as CIRL contains a CUB domain and a serine protease domain, possessing characteristic catalytic triad. C1RL is upregulated in glioblastoma (GBM) and has been proved to be a prognostic marker in hepatocellular carcinoma and renal cell cancer (PMID: 32993564). C1RL has a calculated molecular weight of 53 kDa, and this antibody can detect a band at 70 kDa due to possible N-glycosylation (PMID: 15358180).

Protocols

Product Specific Protocols
WB protocol for C1RL antibody 24820-1-APDownload protocol
IF protocol for C1RL antibody 24820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols