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  • Featured Product
  • KD/KO Validated

RNF167 Polyclonal antibody

RNF167 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24618-1-AP

Synonyms

5730408C10Rik, LP2254, ring finger protein 167, RING105, RNF167

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using RNF167 Polyclonal antibody (24618-1-AP)

    WB analysis of SH-SY5Y using 24618-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using RNF167 Polyclonal antibody (24618-1-AP)

    WB analysis of mouse testis using 24618-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RNF167 Polyclonal antibody (24618-1-AP)

    WB analysis of mouse brain using 24618-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24618-1-AP is RNF167 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF167 fusion protein Ag18383
Full Name ring finger protein 167
Calculated Molecular Weight 350 aa, 38 kDa
Observed Molecular Weight 46-50 kDa
GenBank Accession NumberBC010139
Gene Symbol RNF167
Gene ID (NCBI) 26001
RRIDAB_2879642
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF167 also named as, ring finger protein 167 or LP2254, is a 350 amino acid protein, which contains one protease associated domain and one Ring-type zinc finger. RNF167 as single-pass membrane protein localizes in the endomembrane system and is strongly expressed in the kidney and liver. RNF167 may act as an E3 ubiquitin-protein ligase,or as a part of the E3 complex, which accepts ubiquitin from specific E3 ubiquitin from ubiquitin conjugation enzymes. RNF167 also may play a role in growth regulation in G1/S transition. The molecular weight of RNF167 is 38 kDa, but we detected a 47 kDa protein by western blot. Our result is similar to the figure of one research paper (PMID:23129617).

Protocols

Product Specific Protocols
WB protocol for RNF167 antibody 24618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

RNF167 activates mTORC1 and promotes tumorigenesis by targeting CASTOR1 for ubiquitination and degradation.

Authors - Tingting Li
  • KD Validated
View Article
humanWB

FASEB J

RNF167-mediated ubiquitination of Tollip inhibits TNF-α-triggered NF-κB and MAPK activation

Authors - Zhenzhen Yan
View Article