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RS1 Polyclonal antibody

RS1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 24430-1-AP

Synonyms

Retinoschisin, retinoschisin 1, RS, RS1, XLRS1

Validation Data Gallery

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  • Western Blot (WB) analysis of rat retina tissue using RS1 Polyclonal antibody (24430-1-AP)

    WB analysis of rat retina using 24430-1-AP

    rat retina tissue were subjected to SDS PAGE followed by western blot with 24430-1-AP (RS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using RS1 Polyclonal antibody (24430-1-AP)

    WB analysis of mouse eye using 24430-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 24430-1-AP (RS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RS1 Polyclonal antibody (24430-1-AP)

    IHC staining of mouse eye using 24430-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24430-1-AP (RS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RS1 Polyclonal antibody (24430-1-AP)

    IHC staining of mouse eye using 24430-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24430-1-AP (RS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RS1 Polyclonal antibody (24430-1-AP)

    IHC staining of mouse eye using 24430-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24430-1-AP (RS1 antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using RS1 Polyclonal antibody (24430-1-AP)

    IF Staining of mouse eye using 24430-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using RS1 antibody (24430-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using RS1 Polyclonal antibody (24430-1-AP)

    IF Staining of mouse eye using 24430-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using RS1 antibody (24430-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inrat retina tissue, mouse eye tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24430-1-AP targets RS1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RS1 fusion protein Ag18885
Full Name retinoschisin 1
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC141638
Gene Symbol RS1
Gene ID (NCBI) 6247
RRIDAB_2879545
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RS1 is also named as XLRS1. RS1 can bind negatively charged membrane lipids, such as phosphatidylserine and phosphoinositides. RS1 is required for normal structure and function of the retina (PMID:19093009). It may play a role in cell-cell adhesion processes in the retina (PMID:27114531). RS1 is high expression in the retina (at protein level) (PMID:10915776, PMID:9326935). It is detected in the inner segment of the photoreceptors, the inner nuclear layer, the inner plexiform layer and the ganglion cell layer. At the macula, it is expressed in both the outer and inner nuclear layers and in the inner plexiform layer (PMID:10915776, PMID:10915776). And RS1 is undetectable in the inner plexiform layers and the inner nuclear layer (PMID:10915776)

Protocols

Product Specific Protocols
WB protocol for RS1 antibody 24430-1-APDownload protocol
IHC protocol for RS1 antibody 24430-1-APDownload protocol
IF protocol for RS1 antibody 24430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Biochem Biophys Rep

Intravitreal injection of new adeno-associated viral vector: Enhancing retinoschisin 1 gene transduction in a mouse model of X-linked retinoschisis

Authors - Yan Sun
View Article
mouseWB

Hum Gene Ther

Intravitreal Delivery of rAAV2-hSyn-hRS1 Results in Retinal Ganglion Cell-Specific Gene Expression and Retinal Improvement in the Rs1-KO Mouse

Authors - Yangyang Zheng
View Article