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FAM48A Polyclonal antibody

FAM48A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24419-1-AP

Synonyms

C13, C13orf19, FAM48A, FP757, p38 interacting protein, P38IP, Protein FAM48A

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using FAM48A Polyclonal antibody (24419-1-AP)

    WB analysis of PC-3 using 24419-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24419-1-AP (FAM48A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using FAM48A Polyclonal antibody (24419-1-AP)

    WB analysis of PC-3 using 24419-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24419-1-AP (FAM48A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using FAM48A Polyclonal antibody (24419-1-AP)

    WB analysis of Jurkat using 24419-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24419-1-AP (FAM48A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FAM48A Polyclonal antibody (24419-1-AP)

    IHC staining of mouse testis using 24419-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24419-1-AP (FAM48A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FAM48A Polyclonal antibody (24419-1-AP)

    IHC staining of mouse testis using 24419-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24419-1-AP (FAM48A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using FAM48A Polyclonal antibody (24419-1-AP)

    IHC staining of human testis using 24419-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24419-1-AP (FAM48A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using FAM48A Polyclonal antibody (24419-1-AP)

    IHC staining of human testis using 24419-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24419-1-AP (FAM48A Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inPC-3 cells, Jurkat cells
Positive IHC detected inmouse testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24419-1-AP targets FAM48A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM48A fusion protein Ag19875
Full Name family with sequence similarity 48, member A
Calculated Molecular Weight 779 aa, 86 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC030686
Gene Symbol FAM48A
Gene ID (NCBI) 55578
RRIDAB_2879537
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM48A also named as C13orf19 or SUPT20H is a 779 amino acid protein, which belongs to the SPT20 family. FAM48A is required for MAP kinase p38 activation during gastrulation and for starvation-induced ATG9A trafficking during autophagy. FAM48A localizes in the nucleus and is highly expressed in testis, moderately in brain and pituitary gland.

Protocols

Product Specific Protocols
WB protocol for FAM48A antibody 24419-1-APDownload protocol
IHC protocol for FAM48A antibody 24419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols