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LARGE Polyclonal antibody

LARGE Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24307-1-AP

Synonyms

KIAA0609, LARGE, LARGE1, like glycosyltransferase, MDC1D

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using LARGE Polyclonal antibody (24307-1-AP)

    WB analysis of mouse kidney using 24307-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24307-1-AP (LARGE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LARGE Polyclonal antibody (24307-1-AP)

    WB analysis of mouse brain using 24307-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24307-1-AP (LARGE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using LARGE Polyclonal antibody (24307-1-AP)

    WB analysis of mouse skeletal muscle using 24307-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24307-1-AP (LARGE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using LARGE Polyclonal antibody (24307-1-AP)

    WB analysis of NIH/3T3 using 24307-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24307-1-AP (LARGE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse kidney tissue, mouse brain tissue, mouse skeletal muscle tissue, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24307-1-AP is LARGE Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LARGE fusion protein Ag19458
Full Name like-glycosyltransferase
Calculated Molecular Weight 756 aa, 88 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC126404
Gene Symbol LARGE
Gene ID (NCBI) 9215
RRIDAB_2879486
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LARGE antibody 24307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols