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EB3 Polyclonal antibody

EB3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 23974-1-AP

Synonyms

EB3, End binding protein 3, MAPRE3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using EB3 Polyclonal antibody (23974-1-AP)

    WB analysis of mouse brain using 23974-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using EB3 Polyclonal antibody (23974-1-AP)

    WB analysis of mouse brain using 23974-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using EB3 Polyclonal antibody (23974-1-AP)

    WB analysis of mouse brain using 23974-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using EB3 Polyclonal antibody (23974-1-AP)

    WB analysis of rat brain using 23974-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 23974-1-AP (EB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using EB3 Polyclonal antibody (23974-1-AP)

    IHC staining of human skeletal muscle using 23974-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using EB3 Polyclonal antibody (23974-1-AP)

    IHC staining of human skeletal muscle using 23974-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using EB3 Polyclonal antibody (23974-1-AP)

    IHC staining of human brain using 23974-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using EB3 Polyclonal antibody (23974-1-AP)

    IHC staining of human brain using 23974-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23974-1-AP (EB3 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EB3 Polyclonal antibody (23974-1-AP)

    IHC staining of mouse brain using 23974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23974-1-AP (EB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using EB3 Polyclonal antibody (23974-1-AP)

    IF Staining of mouse testis using 23974-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EB3 antibody (23974-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman skeletal muscle tissue, human brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23974-1-AP is EB3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EB3 fusion protein Ag21148
Full Name microtubule-associated protein, RP/EB family, member 3
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 30-36 kDa
GenBank Accession NumberBC011557
Gene Symbol EB3
Gene ID (NCBI) 22924
RRIDAB_2879385
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EB3 antibody 23974-1-APDownload protocol
IHC protocol for EB3 antibody 23974-1-APDownload protocol
IF protocol for EB3 antibody 23974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Nat Commun

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Authors - Hai-Qing Tu
View Article
mouseWB

Cell Tissue Res

Filamin-A-interacting protein 1 (FILIP1) is a dual compartment protein linking myofibrils and microtubules during myogenic differentiation and upon mechanical stress

Authors - Valentina Grande
View Article
ratIF

Neural Regen Res

The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons

Authors - Shi-Yan Sun
View Article