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Alpha Internexin Polyclonal antibody

Alpha Internexin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23881-1-AP

Synonyms

66 kDa neurofilament protein, Alpha internexin, Alpha Inx, INA, NEF5, Neurofilament 5, NF 66, NF-66, TXBP 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alpha Internexin Polyclonal antibody (23881-1-AP)

    WB analysis using 23881-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23881-1-AP (alpha-internexin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Alpha Internexin Polyclonal antibody (23881-1-AP)

    IHC staining of human pancreas cancer using 23881-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 23881-1-AP (alpha-internexin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Alpha Internexin Polyclonal antibody (23881-1-AP)

    IHC staining of human pancreas cancer using 23881-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 23881-1-AP (alpha-internexin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Alpha Internexin Polyclonal antibody (23881-1-AP)

    IHC staining of human pancreas cancer using 23881-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 23881-1-AP (alpha-internexin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using Alpha Internexin Polyclonal antibody (23881-1-AP)

    IHC staining of human brain using 23881-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23881-1-AP (alpha-internexin antibody at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using Alpha Internexin Polyclonal antibody (23881-1-AP)

    IHC staining of human brain using 23881-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23881-1-AP (alpha-internexin antibody at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Alpha Internexin Polyclonal antibody (23881-1-AP)

    IHC staining of human pancreas cancer using 23881-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 23881-1-AP (alpha-internexin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23881-1-AP targets Alpha Internexin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Internexin fusion protein Ag20844
Full Name internexin neuronal intermediate filament protein, alpha
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 66-68 kDa
GenBank Accession NumberBC006359
Gene Symbol INA
Gene ID (NCBI) 9118
RRIDAB_2879348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Alpha Internexin antibody 23881-1-APDownload protocol
IHC protocol for Alpha Internexin antibody 23881-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols