• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Filter:
  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using MRGPRD Polyclonal antibody (23640-1-AP)

    IHC staining of mouse small intestine using 23640-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 23640-1-AP (MRGPRD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat dorsal root ganglion tissue using MRGPRD Polyclonal antibody (23640-1-AP)

    IHC staining of rat dorsal root ganglion using 23640-1-AP

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 23640-1-AP (MRGPRD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using MRGPRD Polyclonal antibody (23640-1-AP)

    IF Staining of mouse small intestine using 23640-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using MRGPRD antibody (23640-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using MRGPRD Polyclonal antibody (23640-1-AP)

    IF Staining of mouse small intestine using 23640-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using MRGPRD antibody (23640-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MRGPRD Polyclonal antibody

MRGPRD Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 23640-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, ELISA

Synonyms

MRGD, Mas-related G-protein coupled receptor member D, MAS related GPR, member D, G-protein coupled receptor TGR7, Beta-alanine receptor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Please visit your regions distributor:

Tested Applications

Positive IHC detected inmouse small intestine tissue, rat dorsal root ganglion tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23640-1-AP targets MRGPRD in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRGPRD fusion protein Ag20316 Predict reactive species
Full Name MAS-related GPR, member D
Calculated Molecular Weight 321 aa, 36 kDa
GenBank Accession NumberBC156660
Gene Symbol MRGPRD
Gene ID (NCBI) 116512
RRIDAB_3669414
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8TDS7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRGPRD (MAS-related G-protein coupled receptor, D), also referred to as hGPCR45 or TGR7, was identified as a novel GPCR in murine and human genomes. As for MRGD, its expression was found in dorsal root ganglia (DRG) and co-localized with Vanilloid receptor-1 (VR-1), which is an essential receptor for heat and pain sensation (PMID: 22715397).

Protocols

Product Specific Protocols
IHC protocol for MRGPRD antibody 23640-1-APDownload protocol
IF protocol for MRGPRD antibody 23640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols