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VSX1 Polyclonal antibody

VSX1 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 23566-1-AP

Synonyms

Homeodomain protein RINX, KTCN, PPCD, PPD, RINX, Transcription factor VSX1, visual system homeobox 1, VSX1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse eye tissue using VSX1 Polyclonal antibody (23566-1-AP)

    WB analysis of mouse eye using 23566-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 23566-1-AP (VSX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using VSX1 Polyclonal antibody (23566-1-AP)

    WB analysis of Raji using 23566-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 23566-1-AP (VSX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using VSX1 Polyclonal antibody (23566-1-AP)

    IF Staining of mouse eye using 23566-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using 23566-1-AP (VSX1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse eye tissue, Raji cells
Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23566-1-AP is VSX1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VSX1 fusion protein Ag19222
Full Name visual system homeobox 1
Calculated Molecular Weight 365 aa, 38 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC126228
Gene Symbol VSX1
Gene ID (NCBI) 30813
RRIDAB_2879297
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VSX1(visual system homeobox 1) is a member of the Vsx1 group of vertebrate paired-like homeodomain transcription factors locating to the nucleus. Mutations in the VSX1 gene in keratoconus have been reported in many studies. VSX1 is considered important in ocular development and is particularly involved in the developing cornea (PMID:21139977). And previous studies have identified that Vsx1 participates in regulating retinal progenitor proliferation, differentiation and functional maintenance of bipolar cells (PMID:25150888).It was demonstrated that Vsx1 express in embryonic craniofacial, adult corneal, and adult retinal cDNA libraries.VSX1 mRNA has been found in the outer tier of the inner nuclear layer of the human retina and the cornea (PMID: 21139977).

Protocols

Product Specific Protocols
WB protocol for VSX1 antibody 23566-1-APDownload protocol
IF protocol for VSX1 antibody 23566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols