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CBX6 Polyclonal antibody

CBX6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23426-1-AP

Synonyms

CBX6, chromobox homolog 6, Chromobox protein homolog 6

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CBX6 Polyclonal antibody (23426-1-AP)

    WB analysis of HepG2 using 23426-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23426-1-AP (CBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CBX6 Polyclonal antibody (23426-1-AP)

    WB analysis using 23426-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23426-1-AP (CBX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CBX6 Polyclonal antibody (23426-1-AP)

    WB analysis of HepG2 using 23426-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23426-1-AP (CBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CBX6 Polyclonal antibody (23426-1-AP)

    WB analysis of HepG2 using 23426-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23426-1-AP (CBX6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using CBX6 Polyclonal antibody (23426-1-AP)

    IHC staining of human skin using 23426-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin slide using 23426-1-AP (CBX6 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human skin tissue using CBX6 Polyclonal antibody (23426-1-AP)

    IHC staining of human skin using 23426-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin slide using 23426-1-AP (CBX6 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 23426-1-AP is CBX6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBX6 fusion protein Ag20014
Full Name chromobox homolog 6
Calculated Molecular Weight 412 aa, 44 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC064900
Gene Symbol CBX6
Gene ID (NCBI) 23466
RRIDAB_2737364
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Chromobox domain (Cbx) gene family, consisting of Polycomb and Heterochromatin Protein 1 genes, involves in transcriptional repression, cell cycle regulation and chromatin remodeling [PMID: 17486619]. Cbx6, other named neuronal pentraxin receptor (NPR), first described as a putative integral membrane pentraxin that interacts with neuronal pentraxin 1 and 2, has also been classified as a Pc protein [PMID: 14647293]. NPR was determined to be a member of a distinct pentraxin protein subfamily, and found to form heteropentamer swithNP1 andNP2, followed by release from cell membranes [PMID: 10748068].

Protocols

Product Specific Protocols
WB protocol for CBX6 antibody 23426-1-APDownload protocol
IHC protocol for CBX6 antibody 23426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.

Authors - Xun Huang
View Article