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GLIS1 Polyclonal antibody

GLIS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23138-1-AP

Synonyms

GLI similar 1, GLIS family zinc finger 1, GLIS1, Zinc finger protein GLIS1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using GLIS1 Polyclonal antibody (23138-1-AP)

    WB analysis of Jurkat using 23138-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23138-1-AP (GLIS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using GLIS1 Polyclonal antibody (23138-1-AP)

    WB analysis of Jurkat using 23138-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23138-1-AP (GLIS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using GLIS1 Polyclonal antibody (23138-1-AP)

    IHC staining of mouse ovary using 23138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 23138-1-AP (GLIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using GLIS1 Polyclonal antibody (23138-1-AP)

    IHC staining of mouse ovary using 23138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 23138-1-AP (GLIS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 23138-1-AP is GLIS1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLIS1 fusion protein Ag19214
Full Name GLIS family zinc finger 1
Calculated Molecular Weight 620 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC101799
Gene Symbol GLIS1
Gene ID (NCBI) 148979
RRIDAB_2879216
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GLIS1 antibody 23138-1-APDownload protocol
IHC protocol for GLIS1 antibody 23138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Reprod Sci

Effect of GLIS1 on the Lymph Node Metastasis of Cervical Squamous Carcinoma Based on Transcriptome Analysis

Authors - Jiazhen Huang
View Article