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PGLYRP2 Polyclonal antibody

PGLYRP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23029-1-AP

Synonyms

HMFT0141, PGLYRP2, PGLYRPL, PGRP L, PGRPL, tagL, tagL alpha, tagl beta, TAGL like

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using PGLYRP2 Polyclonal antibody (23029-1-AP)

    WB analysis of human plasma using 23029-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 23029-1-AP (PGLYRP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human plasma using PGLYRP2 Polyclonal antibody (23029-1-AP)

    IP experiment of human plasma using 23029-1-AP

    IP result of anti-PGLYRP2 (IP:23029-1-AP, 4ug; Detection:23029-1-AP 1:1000) with human plasma lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PGLYRP2 Polyclonal antibody (23029-1-AP)

    IHC staining of human liver cancer using 23029-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23029-1-AP (PGLYRP2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PGLYRP2 Polyclonal antibody (23029-1-AP)

    IHC staining of human liver cancer using 23029-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23029-1-AP (PGLYRP2 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman plasma tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23029-1-AP is PGLYRP2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGLYRP2 fusion protein Ag19336
Full Name peptidoglycan recognition protein 2
Calculated Molecular Weight 576 aa, 62 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC136551
Gene Symbol PGLYRP2
Gene ID (NCBI) 114770
RRIDAB_2879199
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peptidoglycan recognition protein 2 (PGLYRP2) is an N-acetylmuramoyl-L-alanine amidase that hydrolyzes peptidoglycan, a ubiquitous component of bacterial cell walls (PMID: 14506276). It is involved in innate immunity. PGLYRP2 is primarily expressed in liver and secreted into blood (PMID: 11461926). The apparent molecular mass measured by SDS-PAGE is lager than calculated mass of 62 kDa, which is likely due to N-glycosylation (PMID: 16054449).

Protocols

Product Specific Protocols
WB protocol for PGLYRP2 antibody 23029-1-APDownload protocol
IHC protocol for PGLYRP2 antibody 23029-1-APDownload protocol
IP protocol for PGLYRP2 antibody 23029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols