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MDC1 Polyclonal antibody

MDC1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22272-1-AP

Synonyms

NFBD1, NFBD 1, MDC 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MDC1 Polyclonal antibody (22272-1-AP)

    WB analysis using 22272-1-AP

    Total HEK-293 cells and HEK-293 nuclear extract lysates were subjected to SDS PAGE followed by western blot with 22272-1-AP (MDC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MDC1 Polyclonal antibody (22272-1-AP)

    WB analysis of HeLa using 22272-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22272-1-AP (MDC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22272-1-AP targets MDC1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mediator of DNA damage checkpoint 1
Calculated Molecular Weight 227 kDa
Observed Molecular Weight~260 kDa
GenBank Accession NumberNM_014641
Gene Symbol MDC1
Gene ID (NCBI) 9656
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.

Protocols

Product Specific Protocols
WB protocol for MDC1 antibody 22272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols