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Bim Polyclonal antibody

Bim Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22037-1-AP

Synonyms

BAM, BCL2L11, BIM, BIM alpha6, BIM beta6, BIM beta7, BimEL, BimL, BOD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Bim Polyclonal antibody (22037-1-AP)

    WB analysis using 22037-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22037-1-AP (BIM, BCL2L11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Bim Polyclonal antibody (22037-1-AP)

    IHC staining of human breast cancer using 22037-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22037-1-AP (BIM, BCL2L11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Bim Polyclonal antibody (22037-1-AP)

    IHC staining of human breast cancer using 22037-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22037-1-AP (BIM, BCL2L11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Bim Polyclonal antibody (22037-1-AP)

    IHC staining of human prostate cancer using 22037-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22037-1-AP (BIM, BCL2L11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Bim Polyclonal antibody (22037-1-AP)

    IHC staining of human prostate cancer using 22037-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22037-1-AP (BIM, BCL2L11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inRAW 264.7 cells, Raji cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22037-1-AP targets Bim in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Bim fusion protein Ag11875
Full Name BCL2-like 11 (apoptosis facilitator)
Calculated Molecular Weight 198 aa, 22 kDa
Observed Molecular Weight 23 and 18 kDa
GenBank Accession NumberBC033694
Gene Symbol Bim
Gene ID (NCBI) 10018
RRIDAB_2878978
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Bim antibody 22037-1-APDownload protocol
IHC protocol for Bim antibody 22037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
View Article
humanWB

Aging (Albany NY)

Extracorporeal shockwave relieves endothelial injury and dysfunction in steroid-induced osteonecrosis of the femoral head via miR-135b targeting FOXO1: in vitro and in vivo studies.

Authors - Xinjie Wu
View Article
humanWB

Virulence

Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.

Authors - Wei Jie
View Article
humanWB

Oxid Med Cell Longev

One-Two Punch Therapy for the Treatment of T-Cell Malignancies Involving p53-Dependent Cellular Senescence

Authors - Yingjie Qing
View Article
mouseWB

Biochem Pharmacol

Inhibition of IRE1 Suppresses the Catabolic Effect of IL-1β on Nucleus Pulposus Cell and Prevents Intervertebral Disc Degeneration in vivo.

Authors - Honglei Kang
View Article
mouseWB

Mol Cells

Sertad1 Induces Neurological Injury after Ischemic Stroke via the CDK4/p-Rb Pathway.

Authors - Jianxiong Li
View Article